Jump to: PSP Workshop Marine Toxins Seminar and Paralytic Shellfish Toxin Training PSP Workshop Hotel
PSP Workshop
Workshop – June 23 (Lectures, Tacoma) and June 24-25 (Lab, Shoreline)
Lab Registration Here | Accommodations Here | Pre-lab Lecture Schedule Here
Paralytic Shellfish Toxins by LC-Fluorescence
Post-column Oxidation Method (“PCOX” – ISSC/NSSP Type IV)
Instructor Jeff van de Riet, CFIA, Canada
Semi-automation of Pre-column Oxidation Method (AOAC 2005.06)
Instructor Stacey Degrasse, FDA, USA assisted by Robert Hatfield, Cefas, UK.
Paralytic shellfish toxins (PSTs) are a family of potent, algal neurotoxins based on the parent compound saxitoxin. PSTs occur worldwide, and are the most heavily monitored marine biotoxins. This special laboratory workshop will give hands on experience on two officially accepted LC Fluorescence methods. Attendees will also learn about automation of sample pretreatment in the course of learning important updates to AOAC official method 2005.06. The lecture portion of the course, held at the Annual Meeting of the Pacific NW Section at University of Puget Sound (UPS, in Tacoma, WA) will give required pre-laboratory instruction on the two methods and will also be available to others attending the Annual Meeting. The laboratory sessions will be held at the Washington State Public Health Lab (PHL) in Shoreline, WA, are only available by advance registration, and on a space-available basis. Students in the laboratory will learn in detail about these two methods from method experts and gain first-hand experience in the lab.
Join us for this unique event! Please contact James_Hungerford@hotmail.com in advance to reserve a laboratory spot.
Marine Toxins Seminar and Paralytic Shellfish Toxin Training
UPS, Tacoma – Pac NW AOAC – Wed., June 23, 1-5 PM
Lab Registration Here | Accommodations Here | PSP Lab Described Here
Description: This is a Marine Toxins Seminar including presentations, Task Force update, and required pre-lab lectures for the PST Workshop. These lectures are open to all attendees at the Annual Meeting in Tacoma. Lab sessions held in Seattle June 24 and 25 are only by separate, advance registration. Our focus this year is on paralytic shellfish toxins. Those taking shuttle to lab course should prepare before attending.
| 1:00 PM – Welcome Jim Hungerford, FDA/ORA, USA |
| 1:05 PM – Moving from Mouse Bioassays to Modern Methods for Marine Biotoxins – Validation and Training Efforts. Ana Gago-Martinez, EU Reference Laboratory on Marine Biotoxins, and Univ. Vigo, Spain |
| 1:20 PM – Overview on Paralytic Shellfish Toxins. Stacey (Etheridge) DeGrasse, FDA/CFSAN, USA |
| 1:35 PM – Progress Towards Sensitive LC-MS/MS Detection of Paralytic Shellfish Toxins in Alaskan Shellfish. Emanuel Hignutt, ADEC, Alaska, USA |
| 1:50 PM –High Throughput Quantitation of Saxitoxin in Human Urine Using 96-well Solid Phase Extraction. Rudy Johnson, CDC, Georgia, USA. |
| 2:10 PM – Marine and Freshwater Toxins – Validations, Implementations, Fluidics Automation, and the Oxidations behind the LC Fluorescence Methods for the Paralytic Shellfish Toxins. Jim Hungerford, FDA/ORA, USA |
| 2:25 PM – Semi Automated, Pre-Column Oxidation Method 2005.06 for PSTs – Monitoring of UK Shellfish. Robert Hatfield, Cefas, UK |
| 2:45 PM – Discussion for 10 min |
| 2:55 PM – Break for 10 min |
| 3:05 PM – Principles of Pre-column Oxidation Method 2005.06 for the Paralytic Shellfish Toxins, Refinements Made at FDA, and Course Overview- (Overview lecture on 2005.06) – Stacey (Etheridge) DeGrasse, FDA/CFSAN, USA |
| 3:45 PM – Instrumentation for Automation of Sample Conditioning. -John de Kanel, Caliper LS, USA |
| 4:00 PM – Detection of PSTs by Post-column oxidation LC Fluorescence – overview of course, chromatogram interpretation, instrument setup and maintenance Distribution of assignments, Jeff van de Riet – CFIA, Canada |
| 4:45 PM Instrumentation for Post-column Derivatization. -Saji George, Pickering Labs, USA |
| 5:00 PM – Laboratory Course Announcements as needed (Shuttle for Edmonds 530 PM) |
PSP Workshop Hotel
Lab Registration Here | Pre-lab Lecture Schedule Here | PSP Lab Described Here
Located on Puget Sound and just north of Seattle, the town of Edmonds is known for its views of the mountains including those on the Olympic Peninsula and the Cascades, a relaxed atmosphere, many boutique shops and excellent restaurants, and a waterfront park. It is also an easy gateway to the beautiful Olympic Peninsula, with its mountains and rainforest, via the neighboring Edmonds Ferry. Seattle is a short ride south. The Edmonds Harbor Inn is near the waterfront and is our workshop hotel.
Hotel Brochure Including Driving Directions Here
Workshop Attendees Must Make Their Own Hotel Reservations
130 W Dayton Street, Edmonds, WA, 98020-4130, United States
$136.46 /night with taxes – reduced rate for workshop
When making reservation refer to “AOAC Workshop”
Phone 425/771-5021 direct for Harbor Inn, Toll free at 800-441-8033
Shuttles to lab included with workshop registration, but only from this location. Directions and map on brochure – link to this above.