Microbiology goes Molecular
PCR Pathratrix Training (On-site at Annual Meeting)
June 22-23, 2010
Jinxin Hu, Ph.D., FDA, Santos Camara, FDA
Marcie van Wart, Matrix MicroScience
Pathatrix is a rapid detection method for pathogens, spoilage bacterium and viruses in food, environmental and bio-threat samples. It uses re-circulating immuno-magnetic separation (RIMS) technology that relies on the use of antibody coated paramagnetic particles to selectively bind and purify the target organism from a comprehensive range of complex matrices. By re-circulating the sample over a “capture phase,” comprised of immobilized antibody coated magnetic beads, this increases the sensitivity of the capture. A high volume wash enables the efficient removal of the sample matrices, non-specific microorganisms and PCR inhibitors. Pathatrix instrument shown here and also diagrams of the steps attaching the capture phase and inserting consumable into the catridge.
In conjunction with real time PCR Pathatrix can provide results within hours. It is also used to improve the performance of other rapid methods such as PCR, lateral flow, ELISA and chromgenic media by reducing or eliminating the need for lengthy pre-enrichment and/or selective enrichment steps.
In this training course, we will discuss the approaches of using antibody coated magnetic particles as well as cationic beads to aid the capture of pathogens from food. After the lecture and discussion, participants will perform a test using Pathatrix involving 4 major steps including sample preparation, pre-incubation, placing the sample in the Pathatrix, and washing and elution. Real time PCR and culture methods will be used to identify the pathogens retrieved from Pathatrix system.
Applied Biosystems Pathogen Detection System for Real-Time PCR Detection of E. coli O157:H7
(On-site at Annual Meeting)
June 23, 2010
Instructors: Sharron Henne, Justin Lao
Applied Molecular Testing Business, Molecular Biology Division
This workshop will provide an overview of the Applied Biosystems Pathogen Detection System and its use for detecting E. coli O157:H7 in food and environmental samples. The workshop will feature:
- An introduction to real-time PCR and its use for pathogen detection
- Manual and automated sample preparation methods for nucleic acids extraction
- A real-time PCR assay for detection of E. coli O157:H7
- Software that fully automates amplification, detection, data collection and analysis
- An onsite demo of the full workflow from sample preparation to data analysis